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NGS regularly delivers breakthroughs in genomics, and to take action, it depends on the essential step of pattern preparation. Complicated and costly workflows are important challenges. At Revvity, we’re addressing the necessity for extra environment friendly and economical NGS options.
Library preparation is a high-stakes course of. Each step, from DNA fragmentation to adapter ligation, measurement choice, and amplification, should be carried out with nice precision to stop errors that might distort sequencing outcomes.
Automated NGS library preparation workflows have emerged as a game-changing resolution to interrupt library prep bottlenecks and enhance sequencing outcomes. By lowering human intervention, automated platforms decrease variability, errors, and pattern loss, delivering reproducible and dependable sequencing-ready libraries.
Usually nonetheless, for laboratory managers and customers, the problem shouldn’t be putting in and beginning to use the automated pattern preparation system, it’s the tough and time-consuming steps to get the automated protocols to create the correct libraries which can be required for sequencing and the technology of high-quality genomic information.
NGS library prep options
The event of vendor-qualified, automated library strategies provides an answer to this rising downside. Revvity pioneered this strategy and has developed over 120 vendor-qualified automated library protocols with further ones constantly in improvement.
Vendor-qualified strategies drastically cut back the necessity for guide programming or customized protocol improvement. These protocols are pre-built, QC-tested, and vendor-approved to fulfill NGS package efficiency requirements with out requiring intensive technique improvement.
The three ranges of automated library preparation readiness
When contemplating the selection and automation of an NGS library preparation package, you will need to perceive the three ranges of automation resolution readiness prior to creating a call.
- Protocol developed; software program coded
Whereas distributors could present {hardware} with pre-written software program protocols for primary DNA/RNA kits, the specialised NGS chemistries require intensive in-house testing and refinement. This “do-it-yourself” strategy necessitates that laboratories deal with protocol optimization, chemistry validation, and software qualification, a course of that may be prolonged and dear, particularly when vendor assist is proscribed in NGS experience.
- Water & chemistry examined with quality-control (QC) outcomes
Automation distributors at this readiness degree conduct thorough in-house testing, together with liquid switch verification, chemistry validation, and QC evaluation. Whereas this reduces the preliminary burden on a laboratory, it doesn’t eradicate it. Laboratories should conduct their very own sequencing validation post-installation. This usually reveals discrepancies necessitating protocol changes, as QC alone can not predict all sequencing outcomes. This iterative course of, involving protocol, chemistry, and library optimization, can lengthen for weeks or months.
- Totally vendor-qualified NGS libraries
For many of its automated protocols, Revvity goes past inside testing by sending ultimate DNA/RNA libraries to NGS package suppliers for sequencing and evaluation. This rigorous course of, culminating in package vendor’s certified standing, confirms that the automated system produces outcomes that meet stringent requirements equal to guide strategies. By providing a “plug-and-play” expertise, vendor-qualified strategies empower labs to maneuver from set up to sequencing in as little as 5 days. Moreover, ongoing collaboration between Revvity and package distributors goals to keep up compatibility when/if kits are up to date.
Price and price range concerns
The true value of an NGS library prep package extends past the instrument and the reagent costs. Laboratories want to contemplate:
- Per-sample value effectivity – Automation reduces labor bills, reagent waste, and batch failures, decreasing long-term prices.
- Vendor-qualified strategies – Pre-validated automation protocols eradicate pricey technique validation and troubleshooting.
Choosing the fitting NGS library prep package is important for reaching high-quality, reproducible sequencing outcomes, nevertheless it turns into much more essential when integrating automation. By selecting vendor-qualified, automation-ready strategies, researchers can tremendously cut back the burden of validation and optimization, fast-tracking sequencing.
At Revvity, we don’t simply present automation options—we’re at your aspect that will help you obtain your NGS objectives.
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